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KMID : 0043319800030010031
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Archives of Pharmacal Research
1980 Volume.3 No. 1 p.31 ~ p.36
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Isolation, Purification and Characterization of Phytohemagglutinating Proteins from Korean Natural Products
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Chung SR
Jeune KH/Kim KA
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Abstract
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Seeds or beans of 55 plants belonging to 31 families were screened by using several different types of red blood cells to find new lectins. In this paper, white kidney bean (Pliaseolits vulgaris C.) was chosen to study biochemical properties of hemagglutinating proteins(lectins). An anion exchanger, DEAF Sephadex A-50, and polyacrylamide disc gel electrophoresis were main techniques used. From three main fractions eluted by stepwise NaCl gradient in 25mM TrisHCl buffer on DEAE Sephadex column, principal lectin was identified.
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KEYWORD
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